MUC1 anticorps

N° du produit ABIN3025207

Cet anticorps anti-MUC1 est un anticorps Souris Monoclonal détectant MUC1 dans WB, FACS, IF et IHC (p). Adapté pour Humain.

Aperçu rapide pour MUC1 anticorps (ABIN3025207)

Antigène: MUC1 (Mucin 1 (MUC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: VU-2G7

Détail du produit anti-MUC1 anticorps

Attributs du produit: mAb VU-2G7 reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of VU-2G7 has not been established with "epitope fingerprinting". The extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

Purification: Protein G affinity chromatography

Immunogène: A synthetic glycosylated MUC1 60mer tandem repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3- COOH was used as the immunogen for the MUC-1 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the MUC-1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (Formalin-paraffin): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the MUC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur MUC1

Antigène: MUC1 (Mucin 1 (MUC1))

Autre désignation: MUC-1

Sujet: MAb VU-2G7 reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of VU-2G7 has not been established with "epitope fingerprinting". The extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

Pathways: Negative Regulation of intrinsic apoptotic Signaling